Journal: Scientific Reports

Article Title: Single-cell transcriptomes reveal heterogeneity of chlorine-induced mice acute lung injury and the inhibitory effect of pentoxifylline on ferroptosis

doi: 10.1038/s41598-023-32093-7

Figure Lengend Snippet: PTX inhibit Cl 2 -induced ferroptosis. ( A , B ) The expression levels of MDA, GSH/GSSG in mice serum treated with Cl 2 , n = 8. ( C , D ) Protein levels of GPX4 and SLC7A11 in mice lung and serum treated with Cl 2 , n = 8. ( E ) GPX4 and SLC7A11 protein levels were determined by western blot analysis, and standardized to the expression of β-actin. Original scans of whole blots are shown in Supplementary information 5. ( F , G ) Immunofluorescence images were used to detect the expression of GPX4 in epithelial and endothelial cells, scale bars 50 μm, n = 6. ( H , I ) Immunofluorescence images were used to detect the expression of SLC7A11 in epithelial and endothelial cells, scale bars 50 μm, n = 6. Epithelial cell: CK8 (green), endothelial cells: Pecam1 (green), GPX4 (red), SLC7A11 (red), cell nuclear (blue). The data was shown as mean ± SEM, * P < 0.05, ** p < 0.01, p < 0.001, **** p < 0.0001, n = 8.

Article Snippet: WB was performed using the following primary antibodies: SLC7A11(orb100617, biorbyt); GPX4 (orb135590, biorbyt); β-Actin (orb625112, biorbyt).

Techniques: Expressing, Western Blot, Immunofluorescence

Journal: PLoS ONE

Article Title: S-Propargyl-Cysteine, a Novel Hydrogen Sulfide Donor, Inhibits Inflammatory Hepcidin and Relieves Anemia of Inflammation by Inhibiting IL-6/STAT3 Pathway

doi: 10.1371/journal.pone.0163289

Figure Lengend Snippet: (A) SPRC and NaHS markedly decreased serum IL-6 levels induced by LPS (n = 5). (B-D) Hepatic Il-6 , Tnfa mRNA levels and JAK2/STAT3 phosphorylation were induced by LPS, and suppressed by SPRC and NaHS. Moreover, PAG diminished the effects of SPRC (n = 5). (E-F) Densitometry analysis of Fig 2D. (G-H) Hepatic mRNA levels of Socs3 and Saa2 , two target genes of STAT3 (n = 5). Representative immunoblots are presented. Whole uncropped images of Fig 2D are shown in . Data are presented as the mean ± SEM. ### p < 0.001 compared with the control group; * p < 0.05, ** p < 0.01, *** p < 0.001, compared with the LPS group; @ p < 0.05, @@ p < 0.01, @@@ p < 0.001.

Article Snippet: Antibody to phospho-JAK2 (Tyr221) was acquired from Bioworld Technology, Louis Park, MN, USA.

Techniques: Western Blot

Journal: PLoS ONE

Article Title: S-Propargyl-Cysteine, a Novel Hydrogen Sulfide Donor, Inhibits Inflammatory Hepcidin and Relieves Anemia of Inflammation by Inhibiting IL-6/STAT3 Pathway

doi: 10.1371/journal.pone.0163289

Figure Lengend Snippet: (A) Serum IL-6 content was not significantly changed by SPRC (n = 7). (B) Hepatic Il-6 mRNA levels were suppressed by SPRC, though only 2-fold change was observed in the model group (n = 7). (C) SPRC successfully ameliorated hepatic JAK2/STAT3 phosphorylation in the turpentine model. (D-E) Densitometry analysis of . Representative immunoblots are presented. Whole uncropped images of Fig 4C are shown in . Data are presented as the mean ± SEM. # p < 0.05, ### p < 0.001 compared with the control group; * p < 0.05, ** p < 0.01, *** p < 0.001 compared with the model group.

Article Snippet: Antibody to phospho-JAK2 (Tyr221) was acquired from Bioworld Technology, Louis Park, MN, USA.

Techniques: Western Blot